How does ADPS™ achieve ultra-high
detection sensitivity?
The Key to The Highest Detection Sensitivity is ADPS™ Smart DNA Polymerase
GENECAST has developed a new DNA polymerase that is directly involved in gene amplification, solving the limitations of detection sensitivity and false positives, a common problem in ctDNA liquid biopsy cancer diagnosis. The new ADPS™ Smart DNA Polymerase is equipped with the ability to distinguish between normal and mutant genes by itself, amplifying only the mutant gene while leaving the normal gene intact.
The effect of ADPS™ Smart DNA Polymerase on Allele Discrimination
Features of ADPS™ Smart DNA Polymerase
DNA Polymerase is an enzyme that recognizes and amplifies the DNA Template-Primer complex. ADPS™ Smart DNA Polymerase can distinguish between a primer being perfectly annealed in a template and a 3 'end mismatch. This innovative discriminative ability drastically improves the detection sensitivity of mutant genes by enabling selective gene amplification. The enhanced detection sensitivity of ADPS™ Smart DNA Polymerase allows for accurate analysis of ctDNA from early cancer patients. Further, ADPS™ Smart DNA Polymerase can also be applied to various molecular diagnostics such as NIPT and Transplantation tests that require discernment.
ADPS™ Smart DNA Polymerase vs conventional DNA Polymerase qPCR experiment
Maximum detection sensitivity, 0.0001%
ADPS™ Smart DNA Polymerase achieved a maximum detection sensitivity of 0.0001%. Through experimentation regarding the BRAF V600E mutant, it was confirmed that the mutant can be quantitatively analyzed in the range of MAFs 100% to 0.0001% based on 3x106 copies.
| Total Number of Gene | BRAF V600E | Mutant Allele Fraction (%) | Ct |
|---|---|---|---|
| 3x106 | 3x106 | 100 | 17.5 |
| 3x106 | 3x105 | 10 | 20.8 |
| 3x106 | 3x104 | 1 | 24.5 |
| 3x106 | 3x103 | 0.1 | 28.1 |
| 3x106 | 3x102 | 0.01 | 31.8 |
| 3x106 | 3x101 | 0.001 | 35.7 |
| 3x106 | 3x100 | 0.0001 | 39.4 |
| NTC (0%) | ND |
Experimental data of ADPS™ Smart DNA Polymerase maximum detection sensitivity
ADPS™ can precisely analyze cancer genes by selectively amplifying cancer genes, while leaving the normal genes intact using the ADPS™ Smart DNA Polymerase. GENECAST’s technology provides a detection sensitivity of 0.0001% without false positives.
Check “How dose ADPS™ operat?”. “What is ADPS™?” to learn more about ADPS™.