ADPS™
smart DNA Polymerase II

ADPS™ Smart DNA Polymerase II is an improved version of DNA polymerase derived from Thermus aquaticus. This enzyme exhibits distinct features, distinguishing between matching and mismatching at the 3' end of primers. In cases of mismatch, it demonstrates significantly reduced amplification efficiency compared to wild-type Taq polymerase.

The unique characteristics of ADPS™ Smart DNA Polymerase II, along with an optimized buffer system, enable high-performance allele-specific PCR without the need for special modifications during the PCR process. Except for allele discrimination ability, all characteristics of the enzyme are the same as those of general Taq polymerase, including polymerase activity and 5’ to 3’ exonuclease activity, but it lacks 3’ to 5’ exonuclease activity.

The utility of ADPS™ Smart DNA Polymerase II extends across a wide range of applications, including SNP genotyping, genetic disease testing, and detecting other genetic alterations. Its versatility spans from variant typing requiring high-sensitivity to ultra-sensitive detection of rare mutations, such as those associated with cancer genes.

ADPS™ Smart DNA Polymerase II

Improved Taq polymerase specifically engineered for allele-specific PCR

Key Feature

• Enzyme and buffer system optimized for allele specific PCR
• Fast extension rate
• Tolerance to inhibitors or salts
• 5' → 3’ exonuclease activity
• Lack of 3' → 5’ exonuclease activity
• GMP-grade manufacturing
• Consistency across production batches

Applications

• Allele-Specific PCR (SNV, In/Del mutation, gene fusion)
• Highly sensitive detection of rare mutation
• SNP genotyping
• Methylation-specific PCR
• NIPT, liquid biopsy, pathogen subtyping, GMO test
• Conventional or Real-Time PCR