BRAF mutation occurs in melanoma and thyroid cancer at a frequency of 30~50%.
4 somatic mutations in the BRAF gene can be detected accurately and quickly with a detection sensitivity of 0.01%.
FAM | V600E mutant (copies) |
gDNA (copies) |
---|---|---|
Red | 30,000 | 0 |
Orange | 3,000 | 27,000 |
Green | 300 | 29,700 |
Blue | 30 | 29,970 |
Violet | 3 | 29,997 |
Black | 0 | 30,000 |
Codon | Mutation | BRAF Nucleic Acid Sequence | COSMIC ID |
---|---|---|---|
600 | V600E1 | 1799T > A | 476 |
V600E2 | 1799_1800TG > AA | 475 | |
V600D1 | 1799_1800TG > AT | 477 | |
V600D2 | 1799_1800TG > AC | 308550 |
Tube | Contents | Quantity |
---|---|---|
BRAF Master Mixture | 400 μL/tube | |
ADPS™ Smart DNA Polymerase | 20 μL/tube | |
BRAF Positive Control (3x104 copies / 5 μL) |
25 μL/tube | |
Nuclease-Free Water | 1.0 mL/tube |
B-Raf inhibitors | BRAF Mutations |
---|---|
Vemurafenib, Dabrafenib | V600 mutations (ex V600E, V600D) |
-The maximum detection sensitivity of ADPS™ BRAF Mutation Test kit v1 is 0.01% (3/30,000), so up to 99 ng of DNA can be used per reaction based on the table below.
Tube | DNA amount / reaction | ||
---|---|---|---|
1 ng | 10 ng | 99 ng | |
Input DNA (copies) | 300 | 3,000 | 30,000 |
Limit of detection (copies) | 3 | 3 | 3 |
Detection sensitivity (maximum) | 1% | 0.10% | 0.01% |
PC | Sample 1 |
Sample 9 |
Sample 17 |
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NTC | Sample 2 |
Sample 10 |
Sample 18 |
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Sample 3 |
Sample 11 |
Sample 19 |
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Sample 4 |
Sample 12 |
Sample 20 |
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Sample 5 |
Sample 13 |
Sample 21 |
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Sample 6 |
Sample 14 |
Sample 22 |
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Sample 7 |
Sample 15 |
Sample 23 |
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Sample 8 |
Sample 16 |
Sample 24 |
1.24 samples can be tested per 1 plate running.
-MMX : Multiplex Mix -PC : Positive Control -NTC : No-Template Control (Negative control)