COVID-19 Subtyping v3
SMARTROL™ SARS-CoV-2 Subtyping Kit v3 (RUO)
Detects Delta variant (B.1.617.2) and 12 more.
Able to distinguish 13 variants of SARS-CoV-2 S gene (N501Y, E484K, K417N, F888L, P681R, L452R) by detecting them with high detection sensitivity.
The test results are should not be used for in-vitro diagnostic use.
This kit can be used for research and tracking management of COVID-19.
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Intended Use
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The SMARTROL™ SARS-CoV-2 Subtyping Kit v3 is a Real- Time Multiplex Reverse Transcriptase (RT) Polymerase Chain Reaction (PCR) test intended for the qualitative determination of S gene mutations (N501Y, E484K, K417N, F888L, P681R and L452R) of the SARS-CoV-2 in [nasopharyngeal (NP) and oropharyngeal (OP) swabs, sputum] collected by a healthcare worker.
The SMARTROL™ SARS-CoV-2 Subtyping Kit v3 is intended for research use only.
DO NOT USE for in-vitro diagnostic use. -
Targeted genes
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US classification
(2021.06.15)Reaction 1 Reaction 2 FAM VIC TexasRed FAM VIC Texas Red Pango
LineageFirst WHO N501Y E484K K417N F888L P681R L452R Detected Label Variant of
ConcernB.1.1.7 U.K. Alpha O B.1.351 South africa Beta O O O P.1 Brazil Gamma O O B.1.427 California Epsilon O B.1.429 California Epsilon O B.1.617.2 India Delta O O Variant of
InterestB.1.525 New York Eta O O B.1.526 New York Iota O B.1.526.1 New York - O P.2 Brazil Zeta O B.1.617 India - O O B.1.617.1 India Kappa O O B.1.617.3 India - O O -
Key Features
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- Target variantsDetection of 13 variants
- Carry-over contamination preventionUNG (Uracil-DNA N-glycosylase) system
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High Detection Sensitivity (LoD)
- N501Y, E484K, P681R, L452R : 9 RNA copies/reaction
- K417N, F888L : 6 RNA copies/reaction - High specificityNo cross-reactivity (31 pathogens)
- High reproducibilityCV of Ct value < 3%
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Product information
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- Catalog No.R9002CRA (24 tests) | R9002CRD (100 tests)
- TechnologyRT-qPCR
- Instrument compatibilityCFX 96, AB 7500/7500 Fast, QuantStudio 5
- Turnaround Time 2 hrs
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Color Contents R9002CRA R9002CRD 2X One-step RT-qPCR MMX (No ROX) 800 μL/tube × 1 1.2 mL/tube × 2 10X Primer / Probe mix 1
(N501Y, E484K, K417N)80 μL/tube × 1 240 μL/tube × 1 10X Primer / Probe mix 2
(F888L, P681R, L452R)80 μL/tube × 1 240 μL/tube × 1 SARS-CoV-2 Variants Positive Control v3 50 μL/tube × 1 50 μL/tube × 1 Nuclease-Free Water 1.0 mL/tube × 1 1.0 mL/tube × 1 -
Verification data
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Target Fluorescence B.1.1.7 B.1.351 P.2 B.1.427 B.1.429 B.1.525 B.1.526 Alpha Beta Zeta Epsilon Epsilon Eta Iota Primer/Probe
Mix 1N501Y FAM E484K VIC K417N Texas Red Primer/Probe
Mix 2F888L FAM P681R VIC L452R Texas Red
