geca™
H-Taq Polymerase

Hot start PCR is a technique used to minimize non-specific amplification by preventing any trace of Taq polymerase activity during PCR setup, which helps improve the specificity, sensitivity, and yield of PCR reactions.

geca™ H-Taq Polymerase, specialized for Hot Start PCR, is a chemically modified form of recombinant Taq polymerase that becomes activated upon heat treatment. The activated enzyme maintains the same functional activities as unmodified Taq polymerase; 5’→3’ polymerase activity, 5’→3’ exonuclease activity, and lack of 3'→5' exonuclease activity.

geca™ H-Taq Polymerase

Key Feature

• Chemically modified Taq polymerase
• Highly specific PCR amplification
• Increased yield of PCR products
• 5’ to 3’ exonuclease activity
• Lack of 3’ to 5’ exonuclease activity
• Consistency across product batches

Applications

• Hot start PCR
• Real-Time PCR (Probe or dye based)
• Multiplex PCR
• TA cloning
• Specific amplification of difficult templates