geca™
N-Taq Polymerase

geca™ N-Taq Polymerase is a thermostable DNA polymerase derived from Thermus aquaticus, expressed and purified in Escherichia coli as a recombinant protein. It is one of the most commonly used thermostable DNA polymerase in PCR DNA amplification reactions.

It possesses 5’→3’ exonuclease activity, making it suitable for Real-Time PCR using hydrolysis probes such as TaqMan probes. Taq polymerase exhibits non-template-dependent activity, which preferentially adds a single adenosine to the 3'-ends of a double-stranded DNA molecule. This activity renders it suitable for specific applications such as TA cloning.

geca™ N-Taq Polymerase

Key Feature

• Recombinant protein in E.coli
• High-performance PCR reaction
• 5’ to 3’ exonuclease activity
• Lack of 3’ to 5’ exonuclease activity
• Consistency across production batches

Applications

• General PCR & Multiplex PCR amplification
• Real-Time PCR (Probe or dye based)
• TA cloning
• DNA labeling reaction
• Primer extension