geca™
Reverse Transcriptase

geca™ Reverse Transcriptase is an engineered form of MMLV Reverse Transcriptase, featuring reduced RNase H activity and increased thermal stability. Its stable activity at high temperatures minimizes the inhibition of the polymerization reaction by RNA secondary structures. These characteristics allow geca™ Reverse Transcriptase to yield higher cDNA levels compared to wild-type enzymes, and to synthesize relatively long cDNA from RNA, resulting in more complete full-length cDNA products. This highly purified recombinant protein, expressed in E. coli, can be utilized across various molecular biology experiments and molecular diagnostic applications.

geca™ Reverse Transcriptase

Key Feature

• RNA-dependent DNA polymerase activity
• Reduced RNase H activity
• Broad range of reaction temperature range (42 ~ 60 ℃)
• Optimal reaction at 50°C
• Increased cDNA Yield, Enhanced cDNA Length Synthesis
• Efficient cDNA Synthesis even from GC-rich RNA
• Consistency across production batches

Applications

• Synthesis of full-length cDNA for libraries or cloning
• One-step RT-PCR for the detection of target RNA sequences
• Rapid amplification of cDNA end (RACE)
• Application in various RNA-based molecular techniques